不同饱血状态微小牛蜱中肠和唾液菌群结构的分析

doi:10.11843/j.issn.0366-6964.2017.03.017

Abstract

Abstract:

The aim of this study was to investigate the difference of bacterial flora and diversity of the saliva and midgut contents of Rhipicephalus microplus with the extension of feeding time, and the proliferation and migration characteristics of dominant genera in saliva and midgut. The saliva (S) and midgut contents (M) collected from partially (P) or fully (F) engorged adult female ticks under sterile environment, the total DNA of bacteria were extracted and PCR amplification. Using Illumina MiSeq sequencing platform, two-terminal sequencing of V3-V4 areas of 16S rDNA was carried. Then OTUs (operational taxonomic units) picking, taxonomy assignment, and diversity analysis were carried on the effective Tags. The results showed that 134 317 sequences were obtained, the total of OTUs were 1 304, OTUs obtained from four samples (SP, SF, MP and MF) were 336, 332, 269, 289, respectively. 152 OTUs had high similarity in 4 samples. Proteobacteria and Firmicutes were the most predominant phyla in all samples. Proteobacteria had higher abundance in midgut than saliva. On the contrary, Firmicutes was highly abundant in saliva. Coxiella, Acinetobacter, Methylobacterium, Brevundimonas, Rickettsia and Escherichia were the major genera. Coxiella and Acinetobacter were highly abundant in all samples. The abundance of Coxiella was higher in partially engorged than fully engorged ticks. Acinetobacter had higher abundance in midgut than saliva. These findings suggested that the saliva and midgut of R. microplus had complicated microbial community structure. The distribution of bacteria were different in different tissues, and the abundance of bacteria changed with the feeding time.

CLC Number:

S852.746

DUAN De-yong，CHENG Tian-yin. Bacterial Flora Analysis of Saliva and Midgut Contents from Partially or Fully Engorged Female Adult Rhipicephalus microplus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 530-537.

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Bacterial Flora Analysis of Saliva and Midgut Contents from Partially or Fully Engorged Female Adult Rhipicephalus microplus

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